std-96-pace

Standard method for running 96 PACE experiments on one plate

Parameters

• Lagoon volume: 500uL
• Cycle time: 30 minutes

Steps

• Adds an on-deck inducer reagent
• 1000uL channels stage tips to be picked up by 96-channel head
• Implements nearly-continuous culture replacement in 96 lagoons
• Samples lagoons into microplate at intervals
• Coordinates with 3rd party plate reader to take luminescence and absorbance measurements
• Automatically bleaches and rinses culture reservoir

Contact

dgretton@mit.edu (maintainer)

erikad@mit.edu

echory@mit.edu

bscwang@mit.edu