FYI: Trimming to region of interest

[LaraMacheriotou]

Dear Team,

I noticed the following in your instructions for training RDP.

"Based on our experience, trimming the sequences to a specific region does not improve accuracy."

I wanted to inform you that I explicitly tested this some years ago, and in my experience trimming did have a significant impact on assignment accuracy.

Metabarcoding free-living marine nematodes using curated 18S and CO1 reference sequence databases for species-level taxonomic assignments, DOI: 10.1002/ece3.4814

I have only ever used trimmed training sets since then and would advise other users to do the same.

Kind regards,

Lara