Error on scglue.genomics.rna_anchored_guidance_graph: not all features are strand specific!

[Freedom04]

Hello, I have the error when running guidance = scglue.genomics.rna_anchored_guidance_graph(rna, atac) when using my dataset.

I will appreciate if you could help me to figure it out. Thanks!

[Jeff1995]

Hi @Freedom04. Thank you for your interest in GLUE! Could you check whether each gene in your rna.var contains a "strand" column with value in "+"/"-"? All genes must have strand information for the guidance graph construction to work.

[peiruzhang]

Hello@Freedom04, have you solved this problem? When I execute guidance = scglue.genomics.rna_anchored_guidance_graph(rna, atac), it prompts "Invalid BED format!". The column names of rna are inconsistent with those of bed. I tried to comment out this step of verification, and then the problem you mentioned above occurred.