hotspots choosing #192
Description
Hello everyone, I’d like to ask how to choose hotspots. Thank you.
My questions are as follows:
When selecting hotspots, should we choose residues or atoms, and which is better for designing effective binders?
How many sites is appropriate to select?
Can the selected sites participate in interactions such as hydrophobic interactions, hydrogen bonds, π–π stacking, etc.?
What are the criteria for choosing hotspots?